| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Unidades Centrais. |
|
Data corrente: |
31/05/2023 |
|
Data da última atualização: |
29/06/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SANTOS, A. S. de O. dos; PEREIRA, H. P.; FOGAÇA, G. N.; MEURER, V. M.; FURTADO, M. A. M.; BORGES, C. A. V.; WELLER, M. M. D. C. A.; MARTINS, M. F. |
|
Afiliação: |
ALESSA SIQUEIRA DE OLIVEIRA DOS SANTOS, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; HYAGO PASSE PEREIRA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; GISELE NOGUEIRA FOGAÇA, UNIVERSIDADE FEDRAL DE JUIZ DE FORA; VANEIDA MARIA MEURER, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; MARCO ANTÔNIO MOREIRA FURTADO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; CRISTIANO AMANCIO VIEIRA BORGES, CNPGL; MAYARA MORENA DEL CAMBRE AMARAL WELLER, UNIVERSIDADE FEDERAL DO ESPÍRITO SANTO; MARTA FONSECA MARTINS, CNPGL. |
|
Título: |
Separation and quantification of milk proteins with the addition of cheese whey by lab-on-a-chip. |
|
Ano de publicação: |
2023 |
|
Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, v. 58, e03099, 2023. |
|
DOI: |
https://doi.org/10.1590/S1678-3921. pab2023.v58.03099 |
|
Idioma: |
Inglês |
|
Notas: |
Título em português: Separação e quantificação de proteínas do leite com adição de soro de queijo por lab-on-a-chip. |
|
Conteúdo: |
ABSTRACT - The objective of this work was to evaluate microfluidic chip electrophoresis, known as lab-on-a-chip technique, for the detection of milk adulteration using cheese whey in comparison with SDS-PAGE. Raw, pasteurized, processed at an ultra-high temperature (UHT), and powdered milk samples received increasing concentrations of cheese whey (0, 1, 2.5, 5, 10, 20, 30, 50, and 100% v/v), and were subjected to lab-on-a-chip electrophoresis and SDS-PAGE to detect their mixtures. The lab-on-a-chip methodology was able to separate and quantify milk proteins. In addition, the tested technique is easy, rapid, sensitive, and can detect the addition of cheese whey in milk from the lowest level tested (1%) for milk proteins a-casein and B-casein. RESUMO - O objetivo deste trabalho foi avaliar a eletroforese com dispositivo de microfluidos, conhecida como técnica lab-on-a-chip, para detecção de adulteração de leite com soro de queijo, em comparação ao SDS-PAGE. Amostras de leite cru, pasteurizado, processado em temperatura ultraalta (UHT) e em pó receberam adição de soro de queijo em concentrações crescentes (0, 1, 2,5, 5, 10, 20, 30, 50 e 100% v/v) e foram submetidas a eletroforese lab-on-a-chip e SDS-PAGE para detectar suas misturas. A metodologia lab-on-a-chip foi capaz de separar e quantificar as proteínas do leite. Além disso, a técnica lab-on-a-chip é fácil, rápida, sensível e pode detectar adição de soro de queijo no leite do nível mais baixo testado (1%) para as proteínas do leite a-caseína e B-caseína. MenosABSTRACT - The objective of this work was to evaluate microfluidic chip electrophoresis, known as lab-on-a-chip technique, for the detection of milk adulteration using cheese whey in comparison with SDS-PAGE. Raw, pasteurized, processed at an ultra-high temperature (UHT), and powdered milk samples received increasing concentrations of cheese whey (0, 1, 2.5, 5, 10, 20, 30, 50, and 100% v/v), and were subjected to lab-on-a-chip electrophoresis and SDS-PAGE to detect their mixtures. The lab-on-a-chip methodology was able to separate and quantify milk proteins. In addition, the tested technique is easy, rapid, sensitive, and can detect the addition of cheese whey in milk from the lowest level tested (1%) for milk proteins a-casein and B-casein. RESUMO - O objetivo deste trabalho foi avaliar a eletroforese com dispositivo de microfluidos, conhecida como técnica lab-on-a-chip, para detecção de adulteração de leite com soro de queijo, em comparação ao SDS-PAGE. Amostras de leite cru, pasteurizado, processado em temperatura ultraalta (UHT) e em pó receberam adição de soro de queijo em concentrações crescentes (0, 1, 2,5, 5, 10, 20, 30, 50 e 100% v/v) e foram submetidas a eletroforese lab-on-a-chip e SDS-PAGE para detectar suas misturas. A metodologia lab-on-a-chip foi capaz de separar e quantificar as proteínas do leite. Além disso, a técnica lab-on-a-chip é fácil, rápida, sensível e pode detectar adição de soro de queijo no leite do nível mais baixo testado (1%) para as proteínas ... Mostrar Tudo |
|
Thesagro: |
Caseína; Eletroforese; Proteína; Soro; Soro de Leite. |
|
Thesaurus Nal: |
Cheese whey; Electrophoresis; Milk proteins; Milk quality. |
|
Categoria do assunto: |
--
L Ciência Animal e Produtos de Origem Animal |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1154143/1/Separation-quantification-milk-2023.pdf
|
|
Marc: |
LEADER 02660naa a2200337 a 4500
001 2154199
005 2023-06-29
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S1678-3921. pab2023.v58.03099$2DOI
100 1 $aSANTOS, A. S. de O. dos
245 $aSeparation and quantification of milk proteins with the addition of cheese whey by lab-on-a-chip.$h[electronic resource]
260 $c2023
500 $aTítulo em português: Separação e quantificação de proteínas do leite com adição de soro de queijo por lab-on-a-chip.
520 $aABSTRACT - The objective of this work was to evaluate microfluidic chip electrophoresis, known as lab-on-a-chip technique, for the detection of milk adulteration using cheese whey in comparison with SDS-PAGE. Raw, pasteurized, processed at an ultra-high temperature (UHT), and powdered milk samples received increasing concentrations of cheese whey (0, 1, 2.5, 5, 10, 20, 30, 50, and 100% v/v), and were subjected to lab-on-a-chip electrophoresis and SDS-PAGE to detect their mixtures. The lab-on-a-chip methodology was able to separate and quantify milk proteins. In addition, the tested technique is easy, rapid, sensitive, and can detect the addition of cheese whey in milk from the lowest level tested (1%) for milk proteins a-casein and B-casein. RESUMO - O objetivo deste trabalho foi avaliar a eletroforese com dispositivo de microfluidos, conhecida como técnica lab-on-a-chip, para detecção de adulteração de leite com soro de queijo, em comparação ao SDS-PAGE. Amostras de leite cru, pasteurizado, processado em temperatura ultraalta (UHT) e em pó receberam adição de soro de queijo em concentrações crescentes (0, 1, 2,5, 5, 10, 20, 30, 50 e 100% v/v) e foram submetidas a eletroforese lab-on-a-chip e SDS-PAGE para detectar suas misturas. A metodologia lab-on-a-chip foi capaz de separar e quantificar as proteínas do leite. Além disso, a técnica lab-on-a-chip é fácil, rápida, sensível e pode detectar adição de soro de queijo no leite do nível mais baixo testado (1%) para as proteínas do leite a-caseína e B-caseína.
650 $aCheese whey
650 $aElectrophoresis
650 $aMilk proteins
650 $aMilk quality
650 $aCaseína
650 $aEletroforese
650 $aProteína
650 $aSoro
650 $aSoro de Leite
700 1 $aPEREIRA, H. P.
700 1 $aFOGAÇA, G. N.
700 1 $aMEURER, V. M.
700 1 $aFURTADO, M. A. M.
700 1 $aBORGES, C. A. V.
700 1 $aWELLER, M. M. D. C. A.
700 1 $aMARTINS, M. F.
773 $tPesquisa Agropecuária Brasileira$gv. 58, e03099, 2023.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
10/08/2004 |
|
Data da última atualização: |
10/04/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
Internacional - A |
|
Autoria: |
SILVA, J. R. V.; HURK, R. van den; COSTA, S. H. F.; ANDRADE, E. R.; NUNES, A. P. A.; FERREIRA, F. V. A.; LOBO, R. N. B.; FIGUEIREDO, J. R. |
|
Afiliação: |
JOSÉ R. V. SILVA, UNIVERSIDADE ESTADUAL DO CEARÁ; ROBERT VAN DEN HURK, UTRECHT UNIVERSITY; SONIA H. F. COSTA, UNIVERSIDADE ESTADUAL DO CEARÁ; EVELYN R. ANDRADE, UNIVERSIDADE ESTADUAL DO CEARÁ; RAIMUNDO NONATO BRAGA LOBO, CNPC. |
|
Título: |
Survival and growth of goat primordial follicles after in vitro culture of ovarian cortical slices in media containing coconut water. |
|
Ano de publicação: |
2004 |
|
Fonte/Imprenta: |
Animal Reproduction Science, v. 81, n. 3/4, p. 273-286, 2004. |
|
DOI: |
10.1016/j.anireprosci.2003.09.006 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: The development of culture systems to support the initiation of growth of primordial follicles is iniportant to the study of the factors that control the earliest stages of folliculogenesis. We investigated the effectiveness of five culture media, two supplements and three culture periods on the survival and growth of goal primordial follicles after culturing ovarian cortex. The media were based on minimal essential minimum (MEM) and coconut water solution (CWS) added in the proportion of 0, 25, 50, 75 or 100%. The two supplements were none versus supplemented with insulin-transferrin--selenium, pyruvate, glutamin (PCNA). The number of follicles in each stage and degenerated follicles were statistically analyzed by ANOVA using a factorial design and the significance of differences assessed using Tukey test. Chi-square test was used to compare the per?ntage of follicles with PCNA positive granulosa cells. As the culture period progressed, the number of primordial follicles fell and there was a significant increase in the number of primary follicles. The fall in the number of primordial follicles was particularly marked after 1 day culture. No effect of media on the number of primordial and primary follicles was observed after culture, but MEM as weil as supplements increased the number of intermediate follicles. Follicular degeneration was kept at the same level after culture in the media tested. except for pure CWS that increased the number of degenerated follicles. ln contrast, addition of supplements to culture media reduced follicular degeneration. ln non-cultured tissue, PCNA was expressed in granulosa cells of 31.6% of the growing follicles. This percentage had not significantly changed after 5 days culture in the various media, indicating the maintenance of proliferation activity of granulosa cens during culture. ln conclusion, it is shown that goat primordial follicles may be successfully activated after in vitro culture in aIl media tested. However, when pure CWS is used the follicular degeneration is enhanced, but the addition of supplements to culture media decrease follicular degeneration. MenosAbstract: The development of culture systems to support the initiation of growth of primordial follicles is iniportant to the study of the factors that control the earliest stages of folliculogenesis. We investigated the effectiveness of five culture media, two supplements and three culture periods on the survival and growth of goal primordial follicles after culturing ovarian cortex. The media were based on minimal essential minimum (MEM) and coconut water solution (CWS) added in the proportion of 0, 25, 50, 75 or 100%. The two supplements were none versus supplemented with insulin-transferrin--selenium, pyruvate, glutamin (PCNA). The number of follicles in each stage and degenerated follicles were statistically analyzed by ANOVA using a factorial design and the significance of differences assessed using Tukey test. Chi-square test was used to compare the per?ntage of follicles with PCNA positive granulosa cells. As the culture period progressed, the number of primordial follicles fell and there was a significant increase in the number of primary follicles. The fall in the number of primordial follicles was particularly marked after 1 day culture. No effect of media on the number of primordial and primary follicles was observed after culture, but MEM as weil as supplements increased the number of intermediate follicles. Follicular degeneration was kept at the same level after culture in the media tested. except for pure CWS that increased the number of degenerated follicles... Mostrar Tudo |
|
Palavras-Chave: |
Activation; Drug effects; Folículo ovariano; Folículo primordial; Growth; Maintenance; Ovary; Primordial follicles; Suplemento; Supplements. |
|
Thesagro: |
Água de Coco; Caprino; Ovário; Reprodução. |
|
Thesaurus NAL: |
Bovine serum albumin; Cell division; Coconut water; Females; Glutamine; Goats; Granulosa cells; Hypoxanthine; In vitro culture; Insulin; Oocytes; Ovarian follicles; Pharmacology; Physiology; Reproduction; Solutions; Transferrin. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 03757naa a2200589 a 4500
001 1530666
005 2024-04-10
008 2004 bl uuuu u00u1 u #d
024 7 $a10.1016/j.anireprosci.2003.09.006$2DOI
100 1 $aSILVA, J. R. V.
245 $aSurvival and growth of goat primordial follicles after in vitro culture of ovarian cortical slices in media containing coconut water.$h[electronic resource]
260 $c2004
520 $aAbstract: The development of culture systems to support the initiation of growth of primordial follicles is iniportant to the study of the factors that control the earliest stages of folliculogenesis. We investigated the effectiveness of five culture media, two supplements and three culture periods on the survival and growth of goal primordial follicles after culturing ovarian cortex. The media were based on minimal essential minimum (MEM) and coconut water solution (CWS) added in the proportion of 0, 25, 50, 75 or 100%. The two supplements were none versus supplemented with insulin-transferrin--selenium, pyruvate, glutamin (PCNA). The number of follicles in each stage and degenerated follicles were statistically analyzed by ANOVA using a factorial design and the significance of differences assessed using Tukey test. Chi-square test was used to compare the per?ntage of follicles with PCNA positive granulosa cells. As the culture period progressed, the number of primordial follicles fell and there was a significant increase in the number of primary follicles. The fall in the number of primordial follicles was particularly marked after 1 day culture. No effect of media on the number of primordial and primary follicles was observed after culture, but MEM as weil as supplements increased the number of intermediate follicles. Follicular degeneration was kept at the same level after culture in the media tested. except for pure CWS that increased the number of degenerated follicles. ln contrast, addition of supplements to culture media reduced follicular degeneration. ln non-cultured tissue, PCNA was expressed in granulosa cells of 31.6% of the growing follicles. This percentage had not significantly changed after 5 days culture in the various media, indicating the maintenance of proliferation activity of granulosa cens during culture. ln conclusion, it is shown that goat primordial follicles may be successfully activated after in vitro culture in aIl media tested. However, when pure CWS is used the follicular degeneration is enhanced, but the addition of supplements to culture media decrease follicular degeneration.
650 $aBovine serum albumin
650 $aCell division
650 $aCoconut water
650 $aFemales
650 $aGlutamine
650 $aGoats
650 $aGranulosa cells
650 $aHypoxanthine
650 $aIn vitro culture
650 $aInsulin
650 $aOocytes
650 $aOvarian follicles
650 $aPharmacology
650 $aPhysiology
650 $aReproduction
650 $aSolutions
650 $aTransferrin
650 $aÁgua de Coco
650 $aCaprino
650 $aOvário
650 $aReprodução
653 $aActivation
653 $aDrug effects
653 $aFolículo ovariano
653 $aFolículo primordial
653 $aGrowth
653 $aMaintenance
653 $aOvary
653 $aPrimordial follicles
653 $aSuplemento
653 $aSupplements
700 1 $aHURK, R. van den
700 1 $aCOSTA, S. H. F.
700 1 $aANDRADE, E. R.
700 1 $aNUNES, A. P. A.
700 1 $aFERREIRA, F. V. A.
700 1 $aLOBO, R. N. B.
700 1 $aFIGUEIREDO, J. R.
773 $tAnimal Reproduction Science$gv. 81, n. 3/4, p. 273-286, 2004.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
